HBV感染者外周血淋巴细胞DNA损伤的监测

Survey on DNA Injury in Peripheral lymphocytes of HBV Infection

[目的 ]对HBV感染者外周血淋巴细胞DNA损伤进行监测。 [方法 ]采用单细胞凝胶电泳法 ,在荧光显微镜下观察淋巴细胞损伤情况。 [结果 ] 5 6名HBV感染者外周血淋巴细胞DNA损伤率为 2 4 1% ,其中急性感染者为2 8 5 % ,慢性感染者损伤率为 2 2 5 % ,携带者损伤率为 18 6% (P <0 0 0 1) ;82名健康者的损伤率为 10 3 % (与感染者比较P <0 0 0 1)。[结论 ]HBV感染可以造成外周血淋巴细胞DNA损伤 ,且不同感染模式的损伤情况不同。

Objective\] To survey DNA injury in peripheral lymphocyte of HBV infection persons \ Lymphocyte injury was observed with fluorescence microscope by the method of single cell gelatin electrophoresis (SCGE) \ The rate of DNA injure in peripheral lymphocyte in 56 HBV infected person was 24 1% in which the rate of acute and chronic HBV infected persons were 28 5% and 22 5% respectively And the rate of HBV carriers was 18 6% (P<0 001) The DNA injury rate of 82 health persons was 10 3% and lower than the rate of HBV infected persons (P<0 001) \[Conclusion\] DNA of peripheral lymphocyte can be injured when HBV infected and the injuries are different from infection types The detecting of DNA injury in peripheral lymphocyte is a reliable method for evaluating HBV prognosis and efficacy for treatment