摘要
高速毛细管电泳技术 (HSCE)的主要特点在于通过增大分离电压和缩短毛细管 ,将分析速度提高到几秒至几分钟内。采用高速毛细管电泳电导法对测定茶碱的血药浓度进行研究 ;优化选择缓冲介质、毛细管长度和内径、分离电压等实验参数 ,对从不同方面来提高分析速度进行初步探讨。结果表明 :茶碱在 6 0s内可以得到较好的分离测定 ,线性范围为 37 85～ 0 15 μg mL ,最小检出浓度为 0 0 8μg mL。此法快速、简便、灵敏 ,耗费低廉 ,符合临床监测血药浓度的要求。
The characteristic of high-speed capillary electrophoresis (HSCE) lies in the fe asibility to improve the separation speed within several seconds or minutes by i ncreasing the voltage and shortening the length of the capillary. A new method o f determination of theophylline in serum by HSCE with conductivity detection was described. Effects of several important factors were investigated. Un der the optimized conditions,theophyline was eluted in 60 seconds with the linea r range and the detection limits of 37.85～0.15 μg/mL and 0.08 μg/mL,respe ctively. The method was simple, rapid and highly reliable for clinical drug moni toring of theophylline.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2003年第3期108-110,共3页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家自然科学基金资助项目 ( 2 96 75 0 33)
广东省自然科学基金资助项目 ( 0 0 12 37)
关键词
高速毛细管电泳
电导检测
茶碱
血药浓度
high-speed capillary electrophoresis
conductivity detection
theophylline
clinical drug monitoring
