急性呼吸窘迫综合征患者血清和支气管肺泡灌洗液中白介素-1β的变化

Changes in interleukin-1β in serum and bronchoalveolar lavage fluid in the patient with acute respiratory distress syndrome

目的 :探讨白细胞介素 1β(IL 1β)在急性呼吸窘迫综合征 (ARDS)发病中的作用。方法 :用酶联免疫吸附试验检测了 13例 ARDS患者和 9例健康者支气管肺泡灌洗液 (BAL F)和血清中 IL 1β含量。结果 :ARDS患者 BAL F及血清中 IL 1β含量〔分别为 (14 4 .6 7± 80 .79) ng/ L 和 (4 5 .71± 7.0 9) ng/ L〕明显高于对照组〔分别为 (2 0 .39± 1.87) ng/ L 和 (35 .0 6± 5 .4 6 ) ng/ L,P=0 .0 0 0和 P=0 .0 0 1〕,且 ARDS患者 BAL F中IL 1β含量也明显高于血清 (P=0 .0 0 0 )。结论 :IL 1β参与了 ARDS的发病过程 ,早期测定 ARDS患者肺内细胞因子可能比血液或血浆中更有价值。

Objective: To study the role of interleukin1β(IL1β) in the pathogenesis of acute respiratory distress syndrome (ARDS). Methods: The bronchoalveolar lavage fluid(BALF)and serum in 13 patients with ARDS and 9 healthy controls were collected. The contents of IL1β were measured by enzymelinked immunoadsorbent assay(ELISA). Results: The levels of IL1β in BALF 〔(144 67±80 79)ng/L〕 and serum 〔(45 71±7 09)ng/L〕 in patients with ARDS were significantly higher than those in the control groups 〔(20 39±1 87)ng/L, P =0 000; (35 06±5 46)ng/L, P =0 001, respectively〕, and the levels of IL1β in BALF were also significantly higher than those of the serum in the patients with ARDS ( P =0 000). Conclusion: The results indicate that IL1β is involved in the pathogenesis of ARDS. Early determination of IL1β level in BALF may be more valuable in the evaluation of disease process of ARDS.