不明原因不孕妇女分泌期子宫内膜Hoxa-10mRNA表达意义的研究

Expression of Hoxa-10 mRNA in the Endometrium of Women With Unexplained Infertile

目的 :探讨不明原因不孕与子宫内膜Hoxa 10基因表达的关系。方法 :采用核酸原位杂交技术 ,以Hox a 10mRNA寡核苷酸为探针进行检测。结果 :不明原因不孕组分泌早中期子宫内膜上Hoxa 10mRNA表达的灰度阳性单位 (PU)值无显著差异 ;正常生育组分泌中期子宫内膜上Hoxa 10mRNA表达的PU值显著高于分泌早期 ;不孕组分泌中期子宫内膜上Hoxa 10mRNA表达的PU值显著低于同期正常组。结论 :子宫内膜Hoxa 10基因在分泌中期的高表达可能和子宫内膜容受力密切相关 ,其表达缺陷可能是导致不明原因不孕的重要原因之一。

Objective: Our aim was to study the relationship between unexplained infertility(IF) and the expression of Hoxa-10 gene in the secretory endometrium.Methods: We determined Hoxa-10 mRNA in the endometria of 28 cases of IF without obvious causes with in situ hybridization technique.Results: In the IF group, the positive units (PU) of the expression of Hoxa-10 mRNA in early-and mid-secretory endometria had no significiant difference.In the normal fertile group(NF),the PU in mid-secretory endometria were significantly higher than that in early-secretory endometria.The PUs in mid-secretory of IF was significantly lower than that of NF.Conclusion: The high expression of Hoxa-10 mRNA in mid-secretory endometrium was associated with endometrial receptivity. Its defective expression might be one of the major causes of unexplained infertility. 252,S]