克拉维酸高产菌的选育

Breeding of Clavulanic Acid High-Producing Strains

在克拉维酸高产菌的选育过程中,应用解除终产物结构类似物作为选择压力,能有效地除去低效价菌株,提高高产菌株的检出率,较传统筛选法优越.试验表明,以带棒链霉菌Strep tomycesclavuligerusCCRC11518(效价为382.4×10-6g/mL)为出发菌,经紫外线诱变,筛选到一株抗20×10-3g/mL的舒巴坦钠突变株my51,my51菌株产克拉维酸的效价达到834.7×10-6g/mL,比出发菌提高了1.18倍.该菌株连续传代7代,克拉维酸的效价保持稳定.

During selection of clavulanic acid highproducing strain,using relieving analog of production as selection pressure can obviate lowproducing strain and increasing the successful rate of obtaining highproducing strain,it is better than traditional screening approach.Experiment showed that using Streptomyces clavuligerus CCRC11518(with the yield of clavulanic acid being 382.4×10-6g/mL)as parent strain,after UV mutation,the mutant my51 resistant to 20×10-3g/mL of subtam was obtained,the mutant produced 834.7×10-6g/mL of clavulanic acid and increased by 118% compared with the parent strain CCRC11518.The mutant my51 was stable in clavulanic acid after continous subcultures for seven generations.