子宫内膜异位症患者腹腔液中肿瘤坏死因子α及转化因子β_1的测定

Levels of tumor necrosis factor-alpha and transforming growth factor-β_1 levels in peritoneal fluid of patients with endometriosis

目的 :了解子宫内膜异位症发病过程中腹腔液中肿瘤坏死因子α(TNF-α)及转化因子β1 (TGF-β1 )的作用。方法 :采用放射免疫双抗体夹心方法测定了 30例子宫内膜异位症患者及 2 1例非子宫内膜异位症 (内异症 )对照组患者腹腔液中肿瘤坏死因子 α(TNF- α)及转化因子 β1 (TGF- β1 )浓度。结果 :腹腔液肿瘤坏死因子 α(TNF- α)及转化因子 β1 (TGF- β1 )在内异症患者及对照组分别为 期 :(15 34± 2 77) ng/ L,(1.2± 0 .4 ) ug/ L 期 :(2 2 36± 392 ) ng/ L,(0 .8± 0 .3) ug/ L(14 32±5 2 3) ng/ L,(0 .9± 0 .4 ) ug/ L。内异症患者腹腔液中肿瘤坏死因子 α(TNF- α)显著升高 (P<0 .0 5 ) ,转化因子 β1 (TGF- β1 )无改变。结论 :腹腔液中肿瘤坏死因子α(TNF-α)

Objectives: To evaluate the levels of tumor necrosis factor-alpha(TNF-α) and transforming growth factor-β 1(TGF-β 1) in peritoneal fluid on pathogenesis of endometriosis(EM).Methods:The TNF-α and TGF-β 1 levels in peritoneal fluid of 30 endometriosis patients and 21 control patients were determined by radioimmunoassay and enzyme linked immunoabsorbentassay(ELISA). Results: TNF-α and TGF-β 1 levels in peritoneal fluid of endometriosis patients and control patients were (1534±277)ng/L and (1.2±0.4) ug/L respectively,at stages I and Ⅱ,and (2236±392)ng/L and (0.8±0.3)ug/L respectively,at stages Ⅲ and Ⅳ,(1432±523)ng/L,(0.9±0.4)ug/L. TNF-α level in peritoneal fluid in endometriosis patients(P<0.05)was significantly increased,with no change in TGF-β 1 level (P>0.05) between the two groups.Conclusion: TNF-α level in peritoneal fluid plays a role in pathophysiology in endometriosis.