胎盘型谷胱甘肽硫转移酶的检测对肝癌诊断的临床价值

Clinical value of detection of serum glutathione S-transferase-πin the diagnosis of hepatocarcinoma and hepatic tumor

目的 :研究谷胱甘肽硫转移酶 - π(GST- π)在肝癌患者血清中的含量及其用于肝癌的临床价值。方法 :应用酶联免疫吸附测定法 (enzyme linkedimm unosorbent assay,EL ISA)对 5 3例原发性肝癌患者和 10例转移性肝癌患者血清标本的 GST- π含量进行检测。结果 :原发性肝癌和转移性肝癌患者血清 GST- π含量平均值均显著高于正常人血清 GST- π含量平均值 (P <0 .0 1,P<0 .0 1) ,阳性率分别为 90 .6 %、90 .0 %。在高分化肝癌组 GST- π上升程度显著高于中、低分化肝癌组 (P<0 .0 5 )。GST-π的阳性率与肝癌的 TNM分期及患者年龄、肿瘤家族史、饮食习惯和居住地理位置等无关 (P >0 .0 5 )。结论 :血清 GST- π检测对肝癌具有较好的辅助诊断作用 ,特别对原发性肝癌 。

Objective:To evaluate the clinical value of measurement of serum glutathione S-transferase-π (GST-π) in human hepatocarcinoma.Methods:ELISA was used to detect the level of serum GST-π in 53 cases of primary hepatocarcinoma and 10 cases of metastatic liver tumor.Results:The primary hepatocarcinoma and metastatic liver tumor mean serum GST-π levels ere significantly higher than those in normal subjects(P<0.01),with a positive rate of 90.6% and 90% respectively.The GST-π increase in the high differentiated hepatocarcinoma was significantly higher than that in the middle or low differentiated.No significant correlation was observed between GST-π levels and pathological staging ,age,tumor family history,diet habit or living geography location (P<0.05).Conclusion:The serun GST-π level should be considered as a sensitive marker for carcinomas and its detection is helpful to the diagnosis of liver tumor especially primary hepatic carcinoma.