雷公藤内酯醇对成纤维细胞的影响

Effect of triptolide on fibroblast cells

目的 :研究雷公藤内酯醇 (Tri)对成纤维细胞的细胞动力学及形态学影响 ,以探讨其抑制增生性瘢痕的机制。 方法 :用光镜、电镜观察用药前后成纤维细胞的形态学变化 ,用流式细胞仪检测细胞周期和细胞凋亡。 结果 :用药组在光镜下细胞密度较低 ,细胞体细长 ,部分失去正常的细胞形态、细胞质红染 ,并可见典型的凋亡小体 ;电镜下粗面内质网疏松 ,内容物减少 ,并可见各期凋亡细胞。细胞动力学检测 (FCM)示用药组凋亡比例 (AI)明显增加 ,并呈一定的量效关系 ,对细胞周期则无影响。 结论 :Tri促进成纤维细胞的凋亡 。

Objectives:To study the effect of triptolide(Tri) on fibroblast cells in vitro culture, morphologically and kinetically. Methods:Light microscope(LM)and electron microscope(EM)were employed to detect the morphological changes in Tri group. Cell DNA content and apoptosis were assayed by flow cytometry (FCM). Results:In Tri group, fibroblast cells showed less density, slimmer body, abnormally and typical apoptosis under LM, while rarefaction and content of rough endoplasmic reticulum(RER) decrease, and apoptosis in different stages could be seen under EM. In FCM,AI elevated remarkably in Tri groups, and the effect is dose dependent, with cell cycle uninfluenced at all. Conclusions:The mechanism of triptolide on hypertrophic scars is related to its promotion on fibroblast apoptosis and inhibition of collagen excretion.