一氧化氮、总抗氧化能力对大鼠隐睾生殖细胞凋亡的影响

The Effect of Nitric Oxide and Total Antioxide Capacity on the Germ Cell Apoptosis in the Rat Cryptorchid

目的 :探讨一氧化氮 (nitricoxide,NO)、总抗氧化能力 (totalantioxidecapacity ,T AOC)对大鼠隐睾生殖细胞凋亡的影响。 方法 :健康SD雄性大鼠 2 0只 ,日龄 2 2d时复制单侧隐睾模型。生物素 dUTP/酶标亲和素测定法检测睾丸生殖细胞凋亡 ,采用硝酸还原酶法测定睾丸组织中NO含量 ,化学比色法测定睾丸组织中T AOC。 结果 :术后第 7d ,与对侧正常睾丸相比 ,隐睾侧睾丸发生凋亡的生殖细胞数显著增加 (P <0 .0 1) ;T AOC显著下降 ;NO含量显著上升 (P <0 .0 1)。 结论 :实验性大鼠隐睾可导致睾丸生殖细胞凋亡增加 ,且与睾丸组织中NO升高及T

Objectives: To study the effect of nitric oxide (NO) and total antioxide capacity (T-AOC) on the germ cell apoptosis in the cryptorchidism rat. Methods: Immature rats (22 day-olds Sprague Dawley) were selected to make the model of unilateral cryptorchid. Germ cell apoptosis was examined by terminal-deoxynucleotidyl transferase mediated d-UTP nick end labeling (TUNEL). Biochemical parameters (NO,T-AOC) were evaluated with spectrophotometric determination. Results: At the seventh day after the operation, as compared to the normal side testis, the number of apoptotic germ cells increased significantly in the undescended testis (P< 0.01), but the testes weight decreased predominantly; the NO level in the undescended testis was significantly higher than that in the normal side testis, and the T-AOC level was predominantly lower (P< 0.01). Conclusions: Experimentally-induced cryptorchidism can lead to the increase of grem cell apoptosis in rat. The results is closely related to the increase of No level and the decrease of T-AOC. Natl J Androl,2003,9(3):175-177