大鼠睾丸局部热作用对生精细胞凋亡的影响

Effect of Local Testicular Heating on Spermatogenic Cell Apoptosis in Rat

目的 :研究大鼠睾丸局部受热后生精细胞凋亡的情况。 方法 :70只雄性SD大鼠随机分为热处理组 ( 43℃ )和对照组 ( 2 2℃ ) ,分别按睾丸局部处理后 12h、1d、3d、6d、10d、5 0d和 80d分成 7个亚组。应用电镜、流式细胞术和原位末端标记法 (TUNEL) ,检测各亚组大鼠睾丸生精细胞凋亡情况。 结果 :电镜显示热处理各组均出现生精细胞凋亡表现 ;流式细胞术检测发现热处理各组亚单倍体细胞百分率明显升高 (P <0 .0 1) ;TUNEL法显示热处理各组生精细胞凋亡率明显高于对照组 (P <0 .0 1) ,各类生精细胞对热的敏感性不同。 结论 :大鼠睾丸局部受热后生精细胞凋亡增加 ,初级精母细胞受热后最敏感 ,其次为精子细胞和精子 。

Objectives: To study the effect of testicular local heating on spermatogenic cell apoptosis in rat. Methods: Seventy male SD rats were divided into heat treatment group (43 ℃) and control group (22 ℃). Each group was further divided into seven sub-groups respectively according to the time of 12 hours and 1 days, 3 days, 6 days, 10 days, 50 days and 80 days after testicular local treatment . The spermatogenic cell apoptosis in all sub-groups was examined by means of electron microscopy, flow cytometry and terminal deoxynucleotidyl transferase-mediated dUDP-nick end labeling(TUNEL) method. Results: In the groups of heat treatment, spermatogenic cell apoptosis was detected by electron microscopy; flow cytometry showed that the percentage of cells with sub-haploid increased(P< 0.01); the percentage of positive TUNEL cells in the heat treatment groups was higher than that in the control group(P< 0.01). Initiation of spermatogenic cell apoptosis after testicular heating was not random but was highly selective. Conclusions: Local testicular heating could increase the spermatogenic cell apoptosis. The most sensitive cell is spermatocyte. Spermatid and sperm also display apparent changes. Heating can increase the apoptosis of spermatogonia in a long period. Natl J Androl,2003,9(3):170-174