培养条件对牛体外受精胚胎核仁及线粒体发育的影响

Effects of Culture Conditions on the Development of the Nucleolusand Mitochondria of in Vitro Fertilized Bovine Embryos

牛体外受精胚胎分别在SOF +FCS、SOF +BSA和SOF +PVA三种培养系统内进行培养 ,然后分别取三个系统中发育到原核期、2细胞、4细胞、8细胞、桑椹胚和囊胚阶段的胚胎进行透射电镜的观察 ,了解培养系统中血清和BSA的添加与否对胚胎发育过程中核仁和线粒体发育的影响。观察结果表明 :与已报道的体内受精胚胎研究结果相比 ,体外受精胚胎核仁的发育比较迟缓 ,培养系统中血清及BSA的添加与否不会显著影响核仁的发育过程。在SOF +FCS和SOF +BSA培养系统中线粒体的发育明显滞后 ,表明培养系统中血清和BSA的添加可能是造成这种现象的重要原因。

Bovine IVF embryos were cultured in SOFFCS,SOFBSA and SOFPVA respectively.In order to understand the effects of serum supplements or the presence of BSA on the development of embryonic nucleolus and mitochondria,2-cell,4-cell and 8-cell embryos,as well as morulae and blastocysts from each culture system,were collected and prepared for observation with a transmission electron microscope.The results indicated that the development of the nucleous was retarded compared to embryos developing in vivo,and was not affected by serum supplement or the presence or absence of BSA.The development of mitochondria was significantly delayed when IVF embryos were cultured in SOPFCS and SOFBSA.