摘要
应用Westernblot和ELISA方法对纯化的重组蛋白PET 2 8a( + ) Ts87进行免疫学特性鉴定及保护性研究。Westernblot和ELISA结果显示 ,Ts87抗原可被人工感染旋毛虫的兔血清、病猪血清、病人血清及抗Ts87的兔血清所识别。Ts87抗原免疫BABL c小鼠 ,较对照组减虫率为 2 9% ,说明Ts87抗原可作为旋毛虫免疫诊断和疫苗的候选抗原。
The immunological characteristics and protective immunity of the purified recombinant protein Ts87 were analyzed by Western blot and ELISA. The Ts87 antigen could be recognized by infected rabbit sera, infected swine sera, infected human sera and antisera against Ts87 by Western blot and ELISA. The immunoprotective effect of the Ts87 antigen of Trichinella spiralis was studied by a challenge experiment. Compared with a control group, numbers of larvae in the muscle of immunized mice decreased by 29%. The results showed that the Ts87 antigen might be both a promising immunodiagnosis antigen and a vaccine candidate for Trichinella spiralis.
出处
《动物学杂志》
CAS
CSCD
北大核心
2003年第3期52-55,共4页
Chinese Journal of Zoology
基金
美国中华医学会 (CMB) (Parasitology 98- 6 74)
北京市教委科技发展基金 ( 2 0 0 1- 2 0 0 4)
北京市跨世纪优秀人材工程 ( 0 1KJ- 0 91)资助项目
