基于巯基化合物和纳米金自组装技术固定化方法的研究

Study of Antibody Immobilization Based on Au-S and Colloidal Gold SAMs for Immunosensors

该文提出了一种压电免疫传感器新的蛋白的固定化方法。先在压电石英晶体电极表面自组装半胱氨酸,再通过纳米金与氨基的共价键合作用,在巯基自组装膜的氨基表面形成一均匀的纳米金单层膜,实现蛋白质分子(抗IgG)在传感器表面的固定。探讨了半胱氨酸自组装及IgG抗体固定等一系列实验条件及参数对传感器性能的影响。传感器的频率响应和IgG的浓度在0.33～98.91μg/mL范围内具有良好的线性关系。在0.5 mol/L NaCl十0.01 mol/L NaOH溶液中,蛋白质层可容易地被洗脱,使免疫传感器获得再生。

A novel method based on Au - S and Colloidal Gold self - assembly membranes (SAMs) is proposed for immobilizing antibodies (antigens) . Primarily, a cysteine SAM was obtained on the gold electrode of the quartz crystal, then a well - proportioned nano - Au monolayer is formed on the surfaces of amino - group through the covalent bond between nano - Au and amino - group. After self - assembling a cysteine and nano - Au layer on the surface of the quartz crystal, the immobilization of goat - anti - IgG antibody, as an example, is investigated. The effects of experimental conditions about cysteine self - assembly, IgG antibody immobilization et al. on the performances of immunosensor were discussed respectively. The piezoelectric immunosensor can quantitatively determine IgG in the range of 0. 33 - 98. 91 μLg/mL. Moreover, the protein layer can easily be removed in 0. 5 mol/L NaCl + 0. 01 mol/L NaOH solution, making the immunosensor regenerable.