摘要
研制多糖修饰电流型测酚酪氨酸酶碳糊电极。选择了麦芽糊精作为酪氨酸酶的激活剂,采用Nafion 117膜来抗阴离子干扰,聚乙烯亚胺(PEI)作包埋剂以抗阳离子干扰,论文通过正交实验确定了以碳糊电极为基底电极的酪氨酸酶电极的各组分含量为:酪氨酸酶400U,10％麦芽糊精溶液10μL,0.2％PEI溶液10μL,0.5％Nafion 117溶液2μL。所研制生物电极的工作条件为:工作电位-100mV vs.Hg/Hg_(2)Cl_2(s)电极,工作pH为5.40,测量时间1.0min。在此测量条件下,所研制电极对苯酚的检测下限为5.00×10^(-8)mol/L,线性区间为2.00×10^(-7)mol/L-5.00×10^(-5)mol/L,RSD=1.18％,RE=-0.60％。用所研制电极测定炼油废水中酚的含量测定结果令人满意,加标回收率为100.7％。
The polysaccharide modified amprometric biosensor for the determination of phenols based on tyrosinase carbon paste electrode has been prepared in this paper. Maltodextrin was selected as an active agent of tyrosinase and Nafion 117 was used to make a selective membrance on the surface of biosensor, which could decrease the interference of anion. PEI was mixtured with tyrosinase solution, used to prevent the interference of cation. The constituents of the active membrane of the biosensor contain tyrosinase 400 U, 10% Maltodextrin solution 10 μL, 0. 2% PEI solution 10 μL and 0.5% Nafion 117 solution -2 μL. The working potential was - 100mV vs. Hg/Hg2Cl2(s), and working pH was 5. 40. The time for measurement was 1. 0 min. Under these condition, the detection limit for the determination of phenols was 5. 00 × 10-8 mol/L and the linear range was 2. 00 × 10-7 mol/L - 5. 00 × 10-5 mol/L. The precision and the accuracy for standardized solution of phenol were satisfactory. The USD is 1. 18% and the RE is -0. 06%. The biosensor was used to detect the phonelic compound of the oil refinery wastewater with satisfactory results.
出处
《化学传感器》
CAS
2003年第1期49-57,共9页
Chemical Sensors
