老年人血中氧化/抗氧化物检测的临床意义

CLINICAL VALUE OF DETERMINING OXIDANT AND ANTIOXIDANT IN BLOOD OF SENILE PEOPLE

目的 通过测定老年人血中脂质过氧化物 (MDA)、超氧化物歧化酶 (SOD)及总抗氧化能力 (TAOC) ,探讨监测老年人体内氧化 /抗氧化状态的临床意义。 方法 分别用硫代巴比妥法、邻苯三酚自氧化法和Fe3 + /Fe2 + 化学法对 60例老年人及 30例青年人血中MDA、SOD、TAOC进行测定并计算TAOC/MDA比值。结果 老年组血浆MDA浓度明显高于青年组 (P <0 .0 5 ) ,且男性显著高于女性 (P <0 .0 5 ) ;老年男性TAOC浓度明显低于女性 ,SOD活性女性高于男性 ,但无统计学意义 (P >0 .0 5 ) ;相关分析表明 :MDA与SOD、TAOC之间呈显著负相关 (r1=- 0 .82 1,P <0 .0 5 ;r2 =- 0 .74 5 ,P <0 .0 5 ) ,与年龄呈显著正相关 (r =0 .766,P <0 .0 5 ) ;SOD、TAOC及TAOC/MDA与年龄呈显著负相关 (r1=- 0 .790 ,P <0 .0 5 ;r2 =- 0 .713,P <0 .0 5 ;r3 =- 0 .687,P <0 .0 5 )。结论 MDA、SOD、TAOC和TAOC/MDA四项指标对检测老年人机体内氧化 /抗氧化状态。

Objective To explore the clinical value of monitoring the oxidants and antioxidants of in blood of senile people by determining tipid malondialdehyde(MDA),superoxide dismutase (SOD) and total antioxidative capability(TAOC).Methods The MDA,SOD and TAOC were determined and the value of TAOC/MDA was measured in 60 cases of senile cases and 30 young cases by thiobarbiturates,autoxidation of pyrogallol and Fe 3+/Fe 2+ chemical methods.Results The level of MDA was found higher in senile group than young group(P< 0.05)and also higher in males than in females (P<0.05).The level of TAOC was lower and the level of SOD was higher in males than in females,but both showed no significant difference (P>0.05).The correlative analyses showed that MDA was in obvious correlation with SOD and TAOC(r 1=-0.821,P<0.05;r 2=-0.745,P<0.05),but in positive correlation with age(r=-0.766,P<0.05).SOD TAOC and TAOC/MDA were all in negative correlation with age (r 1=-0.790,P<0.05;r 2=-0.713 ,P<0.05;r 3=-0.687 ,P<0.05).Conclusion MDA,SOD,TAOC and TAOC/MDA play an important role in measuring senile oxidation and antioxidation,in preventing senile diseases and in delaying senility.