马尾松Ls-rDNA5’末端序列分析及其系统学意义

Sequencing of the 5' terminal region of Ls-rDNA from Pinus massoniana and the phylogeny of gymnosperm based on that

马尾松Ls-rDNA 5’末端302个核苷酸序列已被确定,与4种裸子植物和4种被子植物及一种绿藻的同源序列进行比较分析,其所构建的Ls-rDNA系统树图表明:传统分类中的裸子植物与被子植物明显成为单系类群;支持裸子植物的两个单系谱支,即苏铁目—银杏目,买麻藤目—松柏类。Ls-rDNA 5’末端部分序列分析在种子植物高等级分类群系统进化研究中具有重要作用。

The 302-nucleotide sequences of the 5' terminal region of Ls-rDNA from Pinus massoniana lamb was determined. The phylogenetic tree based on the data of Ls-rDNA suggested that Pinus was more closely related to Taxodium and Gnetum than to other gymnosperms, the 3 genera constituted a monophyletic group, while Ginkgo and Cycas formed another monophyletic group within Gymnosperms, which might represent the two evolutionary procession of gymnosperms; The monophyly of both Gymnosperms and Angiosperms was supported. The potential of analysis of phylogeny of partial sequences of 5' terminal region of Ls-rDNA of higher level of taxon of seed plants was addressed.