RP-HPLC测定葛根芩连汤有效部位中黄芩苷和葛根素的含量

Quantitative Determination of Baicalin and Puerarin in the Effective Fraction of Gegenqinlian Decoction by RP-HPLC

目的 建立葛根芩连汤有效部位中黄芩苷和葛根素的RP HPLC含量测定方法。方法 采用YWG C18色谱柱 (2 5 0mm× 4 6mm ,10 μm) ,黄芩苷含量测定流动相为甲醇 -水 -磷酸 (47∶ 5 3∶0 2 ) ,流速 :1 0mL/min ,检测波长 :2 80nm ;葛根素含量测定流动相为甲醇 -水 (2 5∶ 75 ) ,流速 :1 0mL/min ,检测波长 :2 5 0nm。结果 黄芩苷平均回收率为 98 61% ,RSD =2 77% (n =5 ) ;葛根素平均回收率为 10 2 4 % ,RSD =2 70 % (n =5 )。结论 所建立的方法简便、准确 。

Objective To establish a RP-HPLC method for quantitatively determining baicalin and puerarin in the effective fraction of Gegenqinlian Decoction. Method The YWG-C 18 column (250 mm×4.6 mm, 10 μm) was used. The mobile phase for determining baicalin was methanol-water-phosphoric acid (47∶53∶0.2) at a flow rate of 1.0 ml/minute, and the detecting wavelength was 280 nm; and the mobile phase for determining puerarin was methanol-water (25∶75) at a flow rate of 1.0 ml/minute, and the detecting wavelength was 250 nm. Results The average recovery of baicalin was 98.61%, and RSD was 2.77% (n=5); and the average recovery of puerarin was 102.4%, and RSD was 2.70% (n=5). Conclusion This method is easy and accurate, and can be used for controlling the quality of the effective fraction of Gegenqinlian Decoction.