复方大黄抗老制剂提高大脑记忆功能机理的实验研究

Experimental Research on the Mechanisms of the Effect of Compound Rhubarb Antiaging Preparation for Enhancing Memory

目的 探讨复方大黄抗老制剂提高大脑记忆功能的机理。方法 对 12月龄、D -半乳糖造型小鼠脑组织中乙酰胆碱 (Ach)、胆碱酯酶 (AchE)及多种过氧化酶进行检测。结果 复方大黄抗老制剂大剂量能使 12月龄小鼠、D -半乳糖造型小鼠大脑皮质过氧化氢酶 (CAT)、谷胱苷肽过氧化酶 (GSH px)活性明显升高 ,AchE活性降低 ,Ach含量明显增加 ;小脑组织中过氧化物歧化酶(SOD)、铜锌过氧化物歧化酶 (CuZn SOD)、锰过氧化物歧化酶 (Mn SOD)酶活性明显升高 ,过氧化脂质 (LPO)含量明显降低。小剂量可使 12月龄小鼠、D -半乳糖造型小鼠大脑皮质CAT酶活性明显升高 ;小脑组织中LPO含量明显降低。结论 复方大黄抗老制剂提高大脑记忆功能 ,与其能提高脑组织CAT、GSH px、SOD酶活性 ,抑制AchE活性 。

Objective To investigate the mechanisms of the effect of Compound Rhubarb Antiaging Preparation (CRAP) for enhancing memory. Method The changes in the levels of Ach and AchE and in the activities of various kinds of peroxydase were detected in the brain in 12-month-old mice and the mice with cerebral malfunction induced by D-galactose. Results CRAP could markedly increase, when given at a large dose to both 12-month-old mice and the mice with cerebral malfunction induced by D-galactose, the activities of cortical CAT and GSH-px, decrease the activity of AchE, and increase the level of Ach (P<0.05 or P<0.01); and also could markedly increase the activities of cerebellar SOD, CuSOD and Mn-SOD, and decrease the level of LPO (P<0.05 or P<0.01). When given at a small dose to both 12-month-old mice and the mice with cerebral malfunction induced by D-galactose, CRAP could remarkably increase the activity of cortical CAT (P<0.05 or P<0.01), and decrease the level of cerebellar LPO (P<0.05 or P<0.01). Conclusion The effect of enhancing memory of CRAP is close related with its actions of increasing the activities of CAT, GSH-px and SOD, inhibiting the activity of AchE, and increasing the level of Ach.