白藜芦醇苷对内毒素休克肺损伤大鼠Th1/Th2失衡的干预作用

The effect of polydatin (PD) on the imbalance of Th1/Th2 type cytokines with endotoxic shock-induced lung injury in rats

目的:探讨白藜芦醇苷对内毒素休克肺损伤大鼠Th1/Th2类细胞因子水平失衡的影响。方法:采用颈静脉注入LPS复制大鼠急性肺损伤模型,ELISA法检测其外周血单个核细胞产生IFN-γ、IL-4水平及IFN-γ/IL-4比值并观察PD对其影响。结果:在体内实验中,PD能使IL-4水平显著升高(P<0.001),使失衡的IFN-γ/IL-4比值接近正常对照组,肺组织病理切片显示肺损伤减轻;在体外实验中,与急性肺损伤对照组比较,IFN-γ、IL-4水平虽无显著差异(P>0.05),但IFN-γ/IL-4比值接近正常对照组(P>0.05)。结论:PD在体内、外实验中,能纠正内毒素休克大鼠体内失衡的Th1/Th2类细胞因子水平,减轻肺损伤。

Objective: To investigate the effect of PD on the imbalance of Th1/Th2 type cytokines with endotoxic shock-induced lung injury in rats. Methods: We made acute lung injury (ALI) model in rats by injecting lipopolysaccharide (LPS) into jugular. Enzyme-linked immunosorbed assay (ELISA) is employed to measure interferon-γ (IFN-γ) level, interleukine-4 (IL-4) level and the ratio of IFN-γ/IL-4, pathological examination is made under optical microscope at the same time to observe the influence of PD on rats. Results: In vivo, PD increased IL-4 levels significantly( P < 0. 001), and made the ratio of IFN-γ/IL-4 close to the degree of normal control group. Pathological examination illustrated that lung injury was relieved. In vitro, as compared with ALJ model group, although IFN-γ level, IL-4 level didn' t change obviously ( P > 0. 05), the ratio of IFN-γ/IL-4 was near to that of normal control group (compared with normal control group P > 0. 05) . Conclusions: PD can redress the balance of Th1/Th2 type cytokines in vivo and in vitro, and relieve the Iung injury induced by endotoxic shock in rats.