侵染人参果的马铃薯M病毒基因组全序列分析

Complete Sequence Analysis on Potato Virus M Infecting Solanum muricatum

测定了侵染人参果的马铃薯M病毒 (PVM Ch)基因组全序列。线状、单链正义RNA全长 852 6bp ,含 6个开读框 (ORF) ,具麝香石竹潜隐病毒属 (GenusCarlavirus)典型结构特征。序列比较表明它与其他PVM分离物各基因核苷酸和编码蛋白氨基酸序列同源性分别为62 5 %～ 97 2 %和 60 9%～ 97 4% ,其中CP基因最保守 ,而TGB3基因变异最大。系统进化树分析表明美国爱达荷州马铃薯分离物 (PVM Id) (AF0 2 3877)为PVM的一个远缘株系 ,而其他 4个PVM分离物的成簇在外壳蛋白 (Coatprotein ,CP)和核酸结合蛋白 (Nucleicacidbindingpro tein ,NABP)区域略有差异。这是PVM在人参果上侵染的首次报道。

The complete sequence of Potato virus M from Solanum muricatum was determined. The liner, single-strand and positive sense genomic RNA was 8526 nucleotides long and six open reading frames were identified. The genome organization was typical feature of genus Carlavirus. Sequence analysis showed that PVM isolates shared 62.5%～97.2% nucleotide and 60.9%～97.4% amino acid identities. The coat protein gene was most conserved and TGB3 gene was greatly variable. Phylogenetic tree analysis suggested that the Idaho potato isolate(PVM-Id) was a distant strain, and other 4 isolates were grouped discriminatively based on CP and NABP amino acid sequences respectively. This is the first report of PVM from Solanum muricatum.