BALB/c、C57BL/6小鼠糖皮质激素效应差异机制研究

The study of glucocorticoid efficiency difference between BALB/c and C57BL/6 mice

目的 观察BALB/c和C5 7BL/ 6两株小鼠的糖皮质激素 (Gc)效应差异 ,并对其分子机制进行初步探讨。方法 采用放免测定、Westernblot和EMSA方法检测两株小鼠即时应激前后血清皮质醇浓度、脑组织糖皮质激素受体 (GR)表达和脑组织GRE结合效率。结果 BALB/c和C5 7BL/ 6小鼠存在Gc GR信号通路效应差异 ,C5 7BL/ 6小鼠应激前、后GRE结合效率 (P <0 .0 1)和GR胞核 /胞浆比值 (P <0 .0 1)均高于BALB/c小鼠 ,但两株小鼠应激前后血清皮质醇浓度 (P >0 .0 5 )和胞浆内GR含量(P >0 .0 5 )相差不显著。结论 GR核转位量导致的GRE结合差异在两株小鼠Gc GR效应差异中扮演了重要角色。

Objective To observe the glucocorticoid efficiency difference between BALB/c and C57BL/6 mice and also to study its molecular mechanism. Methods RIA, Western blot and EMSA methods were used to detect serum concentration of cortisol, expression of glucocorticoid receptor (GR) and glucocorticoid response element (GRE) binding efficiency in brain tissue. Results The different efficiency of Gc GR pathway was confirmed between BALB/c and C57BL/6 mice. GRE binding efficiency and the ratio of nucleus/cytoplasm of GR in the brain tissue of C57BL/6 mice were significantly higher than those of BALB/c mice, while there was no significant difference of the concentration of cortisol and cytoplasmic GR in the brain tissue of the two strains of mice. Conclusion The difference of GR nucleic translocation may play an important role in the different efficiency of Gc GR pathway between BALB/c and C57BL/6 mice via regulating GRE binding.