超排卵周期中PCOS与IGF-Ⅱ和IGFBP-3关系的研究

Study on serum and follicular fluid levels of insulin-like growth factor-Ⅱand insulin-like growth factor binding protein-3 in patients with polycystic ovary syndrome undergoing in superstimulated cycles

目的 探讨IGF Ⅱ和IGFBP 3与PCOS的关系。方法 分别采用放射免疫法 (RIA)、免疫放射测定法 (IRMA)、磁性分离酶联免疫测定法 (EIA)检测卵泡液及用药前后血清IGF Ⅱ、IGFBP 3和E2 水平。结果 用药后PCOS组血清及卵泡液IGF Ⅱ水平明显低于同期正常组血清及卵泡液IGF Ⅱ水平 (P <0 .0 1) ;用药后PCOS组血清及卵泡液IGFBP 3水平明显高于同期正常组血清及卵泡液IGFBP 3水平 (P <0 .0 1) ;正常组卵泡液IGFBP 3水平与卵泡液IGF Ⅱ水平明显呈负相关 (r=- 0 .6 79,P <0 .0 1)。结论 IGF系统的不平衡状态可能参与PCOS患者疾病的发生发展过程。

Objective To investigate the relationship between polycystic ovary syndrome(PCOS)and insulin like growth factor Ⅱ (IGF Ⅱ) and/or insulin like growth factor binding protein 3 (IGFBP 3) in superstimulated cycles.Methods Samples of serum were collected before use of FSH HP at the third day and before pick up oocyte. Follicular fluid (FF) samples were aspirated at oocyte collection after FSH HP stimulation in the same menstrual cycle. Serum and FF levels of IGF II,IGFBP 3 and E 2 were assayed with the use of RIA,IRMA and EIA (Magnetic Solid Phase) respectively.Results After FSH HP stimulation, serum and FF IGF Ⅱ levels in the control group were significantly higher than those in the PCOS group ( P <0.01). However, after stimulating , serum and FF IGFBP 3 concentrations in the patients with PCOS were significantly higher than those in the nomally ovulating women( P <0.01). A negative correlation was found between IGF Ⅱ and IGFBP 3 levels only in control FF( r = 0.679, P < 0.01). Conclusion Both serum and FF IGF Ⅱ and/or IGFBP 3 levels in PCOS were significantly different from those in controls, confirming a possible role of IGF system imbalance in the development of this disease.