乌头植株再生体系的建立

Establishment of plantlet regeneration system for Aconitum carmichaeli

目的 建立乌头高效再生系统 ,短期内获得大量优质种苗。方法 以试管苗叶片为外植体 ,在添加不同激素配比的培养基上培养。结果 培养基 MS+BA1.0 m g/ L+KT0 .5 mg/ L+NAA0 .2 mg/ L 最适合于乌头叶片不定芽的分化 ;培养基 MS+BA1.5 mg/ L+NAA0 .1mg/ L 有利于芽的增殖 ;培养基 MS+BA0 .5 mg/ L+NAA0 .1m g/ L有利于芽的伸长 ;不加激素的 1/ 2 MS培养基有利于根的诱导。结论 采用组织培养方式可进行乌头的快速繁殖 ,使乌头种苗的工厂化生产成为可能。

Object To establish an effective plantlet regeneration system of Aconitum carmichaeli Debx. for the purpose to obtain a large number of high quality seedling in a short time. Methods Leaves in vitro were tried as the explants and cultivated in different media with the various portion of hormones. Results The medium of MS+BA 1.0 mg/L+KT 0.5 mg/L+NAA 0.2 mg/L was the most suitable one for the induction of shoots; the medium of MS+BA 1.5 mg/L+NAA 0.1 mg/L was beneficial to the propagation of shoots; the medium of MS+BA 0.5 mg/L+NAA 0.1 mg/L was good for the elongation of shoots; and the medium of 1/2 MS without any hormone was suitable for the induction of roots. Conclusion Rapid propagation of A. carmichaeli could be achieved by tissue culture and this will lead to the possibility for its seedling in the industrial production.