黄连的毛细管电泳特征指纹图谱研究

Study on fingerprint spectrum of Coptis chinensis by HPCE

目的 建立黄连的高效毛细管电泳指纹图谱。方法 采用毛细管电泳考察了不同批药材季铵碱的保留时间和峰面积的相关性 ,并以小檗碱作为参照物计算了各色谱峰的相对保留时间和峰面积比值。结果 不同批号的黄连样品色谱组分的保留时间和峰面积的相关系数均大于 0 .9990。结论 建立的黄连高效毛细管电泳指纹图谱稳定、可靠 ,可作为黄连的特征指纹图谱。

Object To establish the fingerprint spectrum of Coptis chinensis Franch. by HPCE. Methods The correlation relativity of retention time and peak area of each chromatographic peak in different batches of medicinal materials were explored respectively, and beberine was chosen as the reference to calculate the relative retention time and the ratio of peak area for each chromatographic peak. Results The correlation coefficients of relative retention time and the ratio of each chromatographic peak were all higher than 0.999 0 in different batches of medicinal materials. Conclusion The established fingerprint spectrum is stable and credible, and could be used as characteristic fingerprint spectrum for C. chinensis.