建立中药“对照指纹图谱”的可行性探讨

Study on the Establishing of Reference Fingerprint for the Traditional Chinese Medicine

建立中药“对照指纹图谱”为标准中指纹图谱相似性比较及相似度限值的确定提供参考。以SPSS统计分析软件对人参、黄芩的HPLC色谱数据所代表的指纹图谱相似度及影响相似度的因素进行研究。中药指纹图谱相似度的影响因素由样品所含的主要化学成分所引起 ,相关系数法比夹角余弦法对指纹图谱中小色谱峰变化的反应明显。提出了在中药质量标准中使用“对照指纹图谱”作为中药指纹图谱相似度比较的参照系。

The Traditional Chinese Medicine Fingerprint (TCMF) was established and some scientific help for standard and comparing similarity of TCMF was offered. The HPLC Fingerprint similarities and influence factors of the Ginseng and Scutellaria have been studied with SPSS statistics analysis program. The important influence factors to TCMF are due to the difference of the main contents of TCM, the Cosin method is better for similarity evaluation than Correlation method. This paper suggested that the “Reference Fingerprint” of TCMF can be used to compare the similarity of the TCMF and the similarity of TCMF is determined by the dispersion of TCM samples.