双波长分光光度法测定复方间苯二酚洗剂中间苯二酚的含量

Determination of Resorcinol in Compound Resorcinol Lotion by Dual Wavelength Spectrophotometry

目的 :用双波长分光光度法测定复方间苯二酚洗剂中间苯二酚的含量。方法 :以水为溶剂 ,在273 5nm和266 1nm波长处测定吸收度并计算差值。结果 :间苯二酚在20 32～60 96μg/ml浓度范围内线性关系良好 (r=0 9998) ;平均回收率为99 59 % ,RSD=0 45% (n=9)。结论 :本方法简便、快捷 。

OBJECTIVE:The content of resorcinol in compound resorcinol lotion was determined by dual wavelength specˉtrophotometry.METHODS:Absorbance was determined by using water as the solvent at the wavelength273.5nm and266.1nm.RESULTS:The results showed that the standard curve of resorcinol concentration ranging from20.32μg/ml to60.96μg/ml was in correspondence with Beer’s law.The absorbance difference and the concentration showed a good linear relationship(r=0.9998).The mean recovery of resorcinol was(99.59±0.45)%(n=9).CONCLUSION:This method is simple,quick and acˉcurate.