薄层扫描法测定三九感冒灵冲剂中对乙酰氨基酚及咖啡因的含量

Determination the Contents of Paracetamol and Caffeine in999Ganmaoling Granules With TLC

目的 :用薄层扫描法同时测定三九感冒灵冲剂中对乙酰氨基酚及咖啡因的含量。方法 :以氯仿 -乙酸乙酯 -甲醇 -氨水(15∶4∶3∶0 3)为展开剂 ,采用双波长反射法锯齿扫描。结果 :对乙酰氨基酚平均回收率为99 33 % ,RSD=1 28% ,3批样品的标示量分别为99 02 %、98 48 %、99 23% ;咖啡因平均回收率为100 1 % ,RSD=1 71 % ,3批样品的标示量分别为99 74%、98 57 %、99 24%。结论 :本方法简便、快速 ,测定结果准确 。

OBJECTIVE:To simultaneously determine the contents of paracetamol and caffeine in999Ganmaoling granules with TLC.METHODS:Chloroform-ethylacetate-methanol-annmonia water(15∶4∶3∶0.3)was taken as developing aˉgent.The spots were scanned with CS-930TLC scanner atλ S =245nm,λ R =315nm for paracetamol and atλ S =270nm,λ R =350nm for caffeine.RESULTS:Average recovery of paracetamol was99.33%,RSD=1.28%;Average recovery of caffeine was100.1%,RSD=1.71%;The labelling contents of paracetamol and caffeine in three batches of samples were99.02%,98.48%,99.23%and99.74%,98.57%,99.24%respectively.CONCLUSION:The method is simple,quick and accurate.It can be used for the quality control of999Ganmaoling.