地塞米松在大鼠缺血随意皮瓣中的作用

The study of the effect of dexamethasone on the random rat ischemic skin flaps

目的 :研究局部使用地塞米松对大鼠缺血随意皮瓣的作用。方法 :建立大鼠背部缺血随意皮瓣的动物模型 ,随机分为 2组 ,实验组术后皮瓣下一次给予地塞米松 5mg(用生理盐水稀释至 3ml) ,对照组术后皮瓣下一次给予 0 .9%生理盐水 3ml。均于术后 7d取材 ,测定皮瓣坏死率和组织中丙二醛含量 ,观察大体及病理切片。结果 :实验组皮瓣坏死率和丙二醛含量较对照组显著降低 (P <0 .0 1 )。光镜下 ,实验组的炎性反应较对照组明显减轻。结论 :地塞米松能提高缺血随意皮瓣的成活率 ,大剂量一次性给药的方法是可行的。

Objective:To investigate the effect of dexamethasone on the random ischemic skin flaps. Methods:The dorsal random skin flaps of Wistar rats were used. After the operations, dexamethasone, at the dose of 25mg/kg, was injected under the flaps. Seven days later, the necrosis rate, the content of malondialdehyde (MDA) and microscopic observation were performed. Results:Compared with that of the control group, the necrosis rate and the content of MDA in the dexamethasone group decreased significantly ( P <0.01).Under the microscope, the tissue inflammatory reactions in the experiment group were not so severe as that of the control group.Conclusion:Dexamethasone could improve the survival of the random ischemic skin flaps and it was applicable to give dexamethasone by subcutaneous injection of one large dose.