食管癌幽门螺杆菌L型感染与其血管形成的关系

Relation between helicobacter pylori L-forrn infection and tumor angiogenesis in human esophageal carcinoma

目的探讨食管癌Hp-L型(即球形:Hp)感染与肿瘤血管形成的相关性及Hp-L型对食管癌生长、侵袭和转移等生物学行为的影响。方法应用革兰染色、电镜和免疫组化ABC法,检测98例食管癌和30例对照组(食管癌切缘正常组织)的Hp-L型;用免疫组化SP法检测上述组织的微血管密度(MVD)值和血管内皮生长因子(VEGF)、p53表达,分析Hp-L型与MVD、VEGF、p53表达以及临床病理因素的关系。结果 食管癌组的Hp-L型阳性率为60.2％。电镜下组织中的Hp-L型有两种类型,可位于癌细胞间或癌细胞胞浆。食管癌组的Hp-L型阳性率、MVD值以及VEGF、p53表达阳性率均高于对照组(P<0.005～0.001),食管癌组中Hp-L型阳性组的MVD值、VEGF和p53表达阳性率也高于其Hp-L型阴性组(P<0.005～0.001)。Hp-L型阳性与MVD呈正相关(r=0.46,P<0.01),与VEGF、p53表达呈正相关(r=0.31,P<0.01)。食管癌组的Hp-L型阳性数与癌细胞的血管侵袭、侵袭深度、食管旁淋巴结转移及远处淋巴结转移相关,而与肿瘤大小无关。结论 Hp-L型与食管癌的血管形成密切相关,是影响食管癌生长、侵袭和转移的重要因素之一。

Objective To investigate the correlation between helicobacter pylori L-form (Hp-L) infection in human esophageal carcinoma (EC) and tumor angiogenesis, and study the effect of Hp-L on the malignant biological behaviors of EC. Methods Hp-L was examined in 98 patients with EC and 30 controls by Gram stain, electronmicroscopic technique and immunohistochemical stain (ABC method) . VEGF, p53 protein and microvessel density (MVD) were examined by immunohistochemical stain (SP method) with their relationship with the clinicopathologic factors analyzed. Results The positive rate of Hp-L was 60.2% in EC group. Two types of Hp-L were delected in the tissue of EC by electronmicroscopic technique, which lay in the outer or inner carcinoma cells. The positive rates of Hp-L, MVD, VEGF and p53 in the cancer group were significantly higher than those in control group( P < 0.005 - 0.001). The positive rates of MVD, VEGF and p53 in the Hp-L positive group of EC were significantly higher than those in Hp-L negative group( P < 0.005 ~ 0.001). The positive rate of Hp-L was correlated with MVD (r = 0.46, P < 0.01) and the expression of VEGF and p53 ( r = 0. 31, P<0.01). The positive rate of Hp-L in the EC group was correlated with vessel invasion, depth of invasion, metastasis to the para-esophageal and distant lymph nodes except tumor size. Conclusion Hp-L infection in EC is closely related with tumor angiogenesis and may be an important promoting factor in esophageal carcinoma growth, invasion and metastasis.