高效液相色谱法及紫外可见分光光度法测定侧柏叶中黄酮含量的比较

Comparison of HPLC and UV-VIS spectrophotometry for the detection of anthoxanthin in leaves of Platychadus orientalis

目的 :通过对高效液相色谱法 (HPLC)及紫外可见分光光度法测定侧柏叶黄酮进行比较 ,寻找一种较准确、简单的测定侧柏叶黄酮的方法。方法 :HPLC法 :流动相由乙晴∶ 0 .0 1mol/ L KH2 PO4 ∶乙酸 (2 8∶ 72∶ 1.5 ,V/ V)组成 ;流速 1.0 m L·min- 1 ;检测波长为2 60 nm,以槲皮甙为参照品。紫外 /可见分光光度法 :以芦丁为参照品 ,410 nm波长测定黄酮含量。结果 :两种方法都有较好的精密度 ,但测定的结果有较大差异 ,紫外 /可见分光光度法测得的结果比 HPLC法高。结论 :以槲皮甙为定量指标更能准确地反映侧柏叶的内在质量 。

Objective:To find a simple method for the detection of anthoxanthin in leaves of Platychadus orientalis. Methods:Anthoxanthin content was determined by HPLC(velocity:1.0 mL/min;wavelength,260 nm)and UV VIS spectrophotometry(wavelength:410 nm),respectively.The contrast samples were quercitrin for HPLC and rutin for UV VIS spectrophotometry.Results:Both methods had high measurement precision,but the measurement result in UV VIS spectrophotometry was about 3 times that in HPLC.Conclusion:Quercitrin as a quantitative indication can precisely reflect anthoxanthin quality.HPLC may be an ideal method for detection of anthoxanthin in leaves of Platychadus orientalis.