摘要
以转入Cu ,Zn-SOD基因的马铃薯为材料 ,研究了在不同NaCl浓度胁迫下其活性氧代谢及保护酶活性的变化。结果表明 ,在NaCl胁迫下转基因马铃薯植株体内的超氧阴离子 (O· -2 )含量明显低于对照非转基因马铃薯 ,H2 O2 含量前者略高于后者但差异不大 ;超氧化物歧化酶活性转基因马铃薯显著高于对照 ,过氧化氢酶及抗坏血酸过氧化物酶活性转基因马铃薯高于对照 ;转基因马铃薯体内的脂质过氧化产物丙二醛 (MDA)含量显著低于对照。研究表明 ,转入Cu ,Zn-SOD基因马铃薯具有较强的抗氧化能力 ,具有耐 (抗 )盐的潜力。
Metabolism of active oxygen and change of cell defense enzyme in potato transfered with Cu, Zn-SOD gene under NaCl stress were studied. The result showed that the transgenic potato had stronger anti-oxidation ability than non-transgenic potato. With the increase of NaCl concentration, the rate of O - 2 generation in transgenic potato was obviously lower than that of non-transgenic potato; the H 2O 2 content of the former was higher than the latter, but discrepancy not large; the MDA content of the latter was higher than the former apparently. The activities of SOD in transgenic potato were higher than non-transgenic potato. The activities of CAT and APX in transgenic potato were higher than non-transgenic potato.
出处
《植物保护》
CAS
CSCD
北大核心
2003年第3期21-24,共4页
Plant Protection
