摘要
目的 研究并阐述三七总皂甙防治冠心病心肌缺血损伤的分子作用机制。方法 应用异丙肾上腺素 (ISOP)复制心肌缺血损伤的动物模型 ,采用光、电镜技术和基因表达芯片技术验证三七总皂甙的作用并探索其分子作用机制。结果 三七总皂甙能够显著减轻异丙肾上腺素所致的大鼠心肌缺血病理损伤程度 ;在基因表达谱的研究中共筛选出 80条差异表达基因 ,其中模型 /正常基因表达谱中筛选出 58条差异表达基因 ,模型 /治疗基因表达谱中筛选出 2 4条差异表达基因。结论 三七总皂甙可能是通过多条途径对多基因的表达进行调控 。
Objective To study the molecular mechanism of panax notoginseng saponins on preventing and treating coronary heart disease.Methods Rat model of myocardial ischemia was established with subcutaneous multi point injecting isoproterenol.Applying the technique of light microscopy,transmission electron microscopy and gene chip to verify the action of the saponin and its mechanism at molecular level.Results Panax notoginseng saponins can significantly decrease the pathological damage,and ameliorate the ultrastructure of myocardial cells of the ischemic rats.The author screened out 58 differential expression genes between isoproterenol induced ischemic rat myocardium and normal rat myocardium in the gene expression profile study;at the same time,screening out 24 differential expression genes between isoproterenol induced ischemic rat myocardium and that of panax notoginseng saponins treated rats.Conclusion The major possible action mechanism of panax notoginseng saponins in prevention and cure of coronary heart disease is to regulate the local expression of related gene through a multi pathway.
出处
《中国药物与临床》
CAS
2003年第3期229-233,共5页
Chinese Remedies & Clinics
基金
国家自然科学基金资助项目(30 10 0 2 38)
