Rho家族蛋白在胃癌细胞系中的表达及意义

EXPRESSION OF Rho FAMILY IN GASTRIC CANCER CELL LINES AND ITS SIGNIFICANCE

为了研究Rho家族 5个重要成员在胃癌细胞中的表达 ,探讨Rho家族在胃癌发生、发展中的意义 ,分别利用半定量RT PCR和Westernblotting检测胃癌细胞系中RhoA、RhoB、RhoC、Rac1和Cdc4 2的mRNA和蛋白表达水平。结果发现 ,与正常对照肠上皮细胞系相比 ,5种胃癌细胞系中的RhoA、Rac1、Cdc4 2的mRNA和蛋白表达均上调 ;在所有细胞系中均无RhoB的表达 ,RhoC仅在胃粘膜组织和有转移潜能的细胞系中有表达 ,而胰岛素可以诱导肠上皮细胞表达RhoB ,进一步证实了RhoB是一个即刻反应蛋白 ;Rho家族中RhoA、Rac1、Cdc4

To study the expression of five important members of Rho family in gastric cancer and investigate the significance of Rho family members in the occurrence and development of gastrointestinal cancer, the mRNA and protein expression levels of RhoA, RhoB, RhoC, Rac1 and Cdc42 in several gastric cancer cell lines were examined by semi quantitative RT PCR and western blotting, respectively. Compared with normal control of gastric mucosa and intestinal epithelial cell line, the mRNA and protein expression levels of RhoA, Rac1 and Cdc42 were up regulated in five gastric cancer cell lines; RhoB protein could not be detected in all cell lines and could be induced by insulin in intestinal epithelial cell line, confirming that RhoB was an immediately induced protein. The increased mRNA and protein expression levels of RhoA, Rac1 and Cdc42 in gastric cancer cell lines indicated that Rho GTPases might play an important role in the carcinogenesis of gastric mucosa.