急性淋巴细胞白血病免疫表型与临床表现及预后的关系

Analysis of immunophenotype of acute lymphoblastic leukemia and clinical presentations and prognosis in children

目的 分析儿童急性淋巴细胞白血病 (ALL)免疫表型与临床表现及预后关系。方法 用流式细胞术对 76例儿童ALL进行免疫分型 ;使用PCR技术探测IgH和TCRδ的重排。 结果 T ALL 8例( 10 .5 3 % ) ,B ALL 65 ( 85 .5 0 % )例 ,T/B混合 3例 ( 0 .0 4% ) ,76例ALL中髓系抗原阳性率为 2 6.3 % ;T ALL与B ALL白细胞数、血红蛋白、血小板、肝脾大和抗原受体基因重排率均无差异 (P <0 .0 5 ) ;CD10 ( - )的B ALL的TCRδ阳性率高于CD10 ( + )的B ALL(P =0 .0 42 ) ;髓系抗原的表达与临床表现及无病生存时间无关 ;T ALL无病生存率低于B ALL(P =0 .0 0 1)。结论 T ALL较B ALL的无病生存时间短 ;髓系抗原的表达与临床表现及无病生存时间无关 ;CD10

Objective To analyze immunophenotype of acute lymphoblastic leukemia and its clinical presentations and prognosis in children. Methods Seventy-six cases of children with acute lymphoblastic leukemia were immunologically classified by flow cytometry and the rearrangement of T cell receptor δ and IgH were detected by PCR.Results Percentage of T-ALL was 10.53 %, while that of B-ALL was 85.50 %, T&B combined cases occupying 0.04 %. The positive rate of myeloid antigen expression was 26.3 %.There was no significant difference among white blood cell count, hemoglobin count , platelet count ,hepatoslenomegaly,and rearrangement of antigen receptor gene(P> 0.05). The positive rate of rearrangement of TCR δ in CD 10 negative B-ALL was higher than that of CD 10 positive B-ALL(P=0.04). There was no association in myoild disease-free survival time with clinical manifestations. The disease-free survival rate of T-ALL patients was lower than that of B-ALL patients (P=0.001). Conclusions The disease-free survival time of T-ALL patients was shorter than that of B-ALL patients. Myoild antigen expression was not associated with clinical manifestations and disease-free survival time. The positive rate of rearrangement of TCRδ in CD 10 negative B-ALL was higher than that of CD 10 positive B-ALL.