不同作用靶点的bFGF反义脱氧寡核苷酸对胶质瘤SWO-38细胞效应的观察

Inhibitory effect of different target-side antisense oligonucleotides on bFGF expression in SWO-38 cells

目的 :比较 3条针对不同位点的碱性成纤维细胞生长因子 (bFGF)反义脱氧寡核苷酸抑制神经胶质瘤SWO - 38细胞bFGF表达的效应。方法 :用脂质体介导bFGF反义脱氧寡核苷酸的转染入SWO - 38细胞 ,MTT及流式细胞术检测细胞增殖及凋亡的改变 ,Western -blot的方法检测bFGF蛋白水平的改变。结果 :3条脱氧寡核苷酸链的细胞增殖抑制率分别为 4 9% ,33% ,5 1% ,促进细胞凋亡率分别为 35 %、2 7%、18% ,对bFGF蛋白的表达降低分别为 6 3%、4 2 %、11%。结论 :对细胞增殖活力、凋亡率、bFGF蛋白水平均有明显效应的脱氧寡核苷酸序列才是理想的反义物质。

AIM: Three different antisense oligonucleotides complementary to basic fibroblast growth factor (bFGF) mRNA were compared in inhibitory effect on gene targeted expression. METHODS: After transfecting bFGF antisense oligonucleotides (asODN) into SWO-38 cells by lipofectin,the proliferation of cells was identified by MTT method, apoptosis was examined by flow cytometric cell cycle analysis and the expression levers of bFGF were detected by Western-blotting. RESULTS: There were 49%,33%,51% inhibition of cell growth and 35%, 27%, 18% cell apoptosis after asODN1, asODN2 and asODN3 treatment.In addition,the decrease in bFGF protein was 63%, 42%, 11%, respectively. CONCLUSION: The data suggeste that asODN1 is a potent target to bFGF mRNA, which inhibits cell growth and induces apoptosis in SWO-38 cells.