从氧自由基、一氧化氮探讨四逆汤抗急性失血性休克的肝脏机制

Liver protection by Sini decoction in hemorrhagic shock and its mechanism relating to oxygen free radical and nitric oxide

目的 :从氧自由基、一氧化氮探讨四逆汤抗急性失血性休克的肝脏机制。方法 :复制急性失血性休克大鼠模型 ,分为假手术对照组 ;单纯休克模型组 ;休克 +生理盐水复苏组 ;休克 +四逆注射液复苏组。四逆注射液(浓度 10 0 0g生药 /L) ,剂量 0 1mL/2 0 0g大鼠。用生理盐水或四逆注射液治疗 3h后处死动物并取组织。测定各组肝脏超氧化物歧化酶 (SOD)活性、丙二醛 (MDA)水平 ,一氧化氮 (NO)水平。采用免疫组化染色法 ,观察诱导型一氧化氮合酶 (iNOS)在肝细胞中的变化特点。RT -PCR观测肝细胞iNOS和内皮源性一氧化氮合酶 (eNOS)基因表达的变化。结果 :模型组在休克 1h后SOD活性明显低于对照组 (P <0 0 1)、MDA水平明显高于对照组 (P <0 0 1)。四逆汤组复苏 3h后肝组织SOD明显高于生理盐水组 (P <0 0 1)、MDA低于生理盐水组 (P <0 0 1)、NO水平明显高于生理盐水组 (P <0 0 1)。生理盐水组iNOS在肝细胞中染色阳性单位明显高于四逆汤组 (P <0 0 5 )。生理盐水组促进iNOSmRNA的表达。四逆汤组eNOSmRNA的表达增强。结论 :四逆汤通过清除氧自由基 ,升高NO ,改善肝组织微循环 ,减少诱导型iNOS表达的各种因素 ,理论上减轻了NO与氧自由基生成的ONOO-的细胞毒作用和血管的低反应性 ,并对肝脏起到保护作用。

AIM: The work was designed to explore protective effects of a traditional Chinese medicine-sini decoction (SD) on liver in hemorrhagic shock and its mechanism relating to oxygen free radical and nitric oxide. METHODS: Anesthetized Wistar rats were subjected to a hemorrhagic shock protocol for 60 min followed by intravenous injection with normal sodium chloride solution or SD solution. Superoxide dismutase (SOD), malondialdehyde (MDA) and nitric oxide (NO) in liver were examined. The inducible nitric oxide synthase (iNOS) was determined immunohistochemically. RT-polymerase chain reaction (RT-PCR)was used to assay the mRNA, which were corresponding to eNOS (endothelial nitric oxide synthase) and iNOS. RESULTS: The activity of SOD decreased, while the concentration of MDA increased in liver during hemorrhagic shock. SD enhanced SOD activity and inhibited a increase in MDA level in liver ( P< 0.01). The NO concentrations in liver in SD group increased at three hours after resuscitation ( P <0.01). In addition, it was found that the expression of iNOS was upregulated in sodium chloride-treated group, while SD upregulated the expression of eNOS. CONCLUSION: SD reduces the liver injury caused by oxygen free radicals during hemorrhagic shock. The increasing NO concentration by SD is through upregulation of endothelial NOS expression.