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深海沉积物样品中古菌的16S rDNA分析 被引量:8

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摘要 利用多管采样器,在太平洋西经177°42′20″,北纬10°35′06″的位置上,从水深5774m的海底获得深海沉积物样本,现场提取DNA进行保存,以此DNA为模板,利用古菌16S rDNA特异引物扩增出样品中古菌的16S rDNA,将扩增所得的16S rDNA进行克隆,建立了该样品中古菌的16S rDNA文库。对16S rDNA扩增片段进行了RFLP分析和序列分析,结果表明这些古菌在系统进化树上的位置靠近,属于Crenarchaeota中的Ⅰ类海洋古菌类群。
出处 《自然科学进展》 北大核心 2003年第6期598-603,共6页
基金 国家重点基础研究发展规划(批准号:G20000078500) 中国大洋专项基金(批准号:4-2-1)
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参考文献16

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