微小病变型肾病患者血尿可溶性白介素2受体的测定及其意义

THE DETERMINATION AND SIGNIFICANCE OF SOLUBLE INTERLEUKIN-2 RECEPTORS IN PLASMA AND URINE OF PATIENTS WITH MINIMAL CHANGE NEPHROTIC SYNDROME

目的 :观察可溶性白细胞介素 2受体 ( s IL- 2 R)在微小病变型肾病综合征 ( MCNS)病人血浆和尿液中浓度变化 ,探讨其在免疫学发病机制中的意义。方法 :用 ELISA分析法检测 2 3例微小病变型肾病综合征患者发病及缓解期血、尿白介素 2受体 ,同时用 3 H- Td R掺入法检测患者淋巴细胞增殖情况。结果 :MC-NS患者在发病期血、尿 s IL- 2 Rα明显增高 ,于缓解期恢复正常。与对照组相比 ,发病期间患者尿液中增高的白介素 2受体与血清浓度不成比例地异常增高。同时尚发现血清可溶性白介素 2受体与 T细胞体外对 PHA的增殖反应呈负相关 ,缓解期恢复正常。结论 :可溶性白介素 2受体可能为血清中抑制 T细胞克隆扩增的一种因子。

Objective:To observe the change of soluble interleukin 2 receptors in plasma and urine of patients with minimal change nephrotic syndrome(MCNS) and to explore their significance in the pathogenesis of MCNS and abnormal immunity.Methods:Plasma and urine concentrations of sIL 2Rα were measured by ELISA in 23 MCNS patients and lymphocytic proliferation with PHA by 3H TdR incoporation assay.Results:The plasma and urine sIL 2R concentration in patitents with MCNS increased and the lymphocytes were in an activated sate in which they secreted some lymphokines.Conclusion:sIL 2R exerted a down modulation on the IL 2 dependent proliferative response.