松乳菇菌种分离及菌丝生长特性的研究

Tissue isolation and mycelium growth characteristics of Lactarius delicious

采用5种培养基配方,对2种不同颜色6个不同来源的松乳菇子实体的不同部位进行组织分离,发现分离松乳菇较好的培养基是PDA培养基,筛选出8株生长较好菌株。对菌株A1进行生长特性研究表明:菌株A1碳源利用范围广,蔗糖最好,葡萄糖次之,果糖最差;对氮源的利用,有机氮单一氮源优于复合氮源,其中尿素不能利用;无机氮源铵态氮优于硝态氮,以硫酸铵效果最好,亚硝酸盐不能利用;对盐浓度适应能力很强,在盐浓度为14g·L-1条件下也具有生长能力,适宜生长的盐浓度范围为0～6g·L-1。生长温度范围为7～37℃,适宜的生长温度是15～30℃,最佳生长温度是27℃。喜中性偏酸性环境,适宜生长的pH值为5～7,最适pH值为6 5。

The tissues on different positions of 6 basidiocarps of different sources are isolated with 5 kinds of culture media.The best medium for tissue isolation of L.delicious is PDA culture medium and 8 welldeveloped mycelia are singled out.The research of growth characteristics of mycelium A1 indicates that the mycelia can absorb all the carbon sucrose tested.The sucrose is the best utilized carbon nutrition,the glucose is the better and the fructose is the worst.As for the utilization of nitrogen source,of organic nitrogen,single nitrogen is better than compound nitrogen;and urea cant be utilized.Of inorganic nitrogen,ammonium nitrogen is better than nitrate nitrogen,ammonium sulfate has the best effect and nitrite cant be utilized.Mycelia have strong adaptability to salt concentration and can grow when the salt concentration is 14 g·L-1.The optimum salt concentration for mycelia growth ranges from 0 to 6 g·L-1.The range of temperature for growth is 7～37 ℃.The optimum temperature for mycelia growth ranges from 15 to 30 ℃.The optimum temperature for growth is 27 ℃.The mycelia prefer neutral and acid environment.The pH values suitable for growth is 5～7 and the optimum one is 65.[Ch,2 fig.4 tab.7 ref.]