VLA-4整联蛋白和造血细胞迁移之间的相关关系研究(英文)

Correlation between VLA-4 Integrin and Hematopoietic Cell Migration

为了阐明VLA 4 (CD4 9d)和造血细胞迁移方向之间的相关关系 ,将重组人G CSF稀释后注射于小鼠皮下 ,以流式细胞仪检测不同时间后Sca 1+ 细胞表面CD4 9d的表达 ,并分析Sca 1+ 细胞计数与CD4 9d表达之间的相关关系。结果表明 ,注射G CSF后骨髓和外周血的CD4 9d的表达都明显下降 ,同时在第 7- 9天外周血Sca 1+ 细胞数达到高峰 ,骨髓有核细胞数下降。而随着CD4 9d的表达回升 ,外周血Sca 1+ 细胞数下降 ,骨髓细胞数却又有增多趋势。结论 :VLA 4介导造血细胞与骨髓微环境的粘附 ,调节CD4

To clarify the relationship between VLA 4 (CD49d) expression and hematopoietic cell migrating direction, mice were injected subcutaneously with diluted rhG CSF for different times. The expressions of CD49d on Sca 1 + cells were examined by flow cytometry. The relations between CD49d expression and Sca 1 + cell enumerations were performed by statistical analysis. The results showed that with the administration of G CSF, the expressions of CD49d in bone marrow (BM) and peripheral blood (PB) declined, meanwhile the number of Sca 1 + cells in peripheral blood reached the peak in sharp contrast to BM nucleated cell number dropping on the seventh to ninth day. When CD49d expression rose again, the PB Sca 1 + cells descended with the rising of BM nucleated cell number. In conclusion, VLA 4 mediates the hematopoietic cell adhesion to BM microenvironment. The regulation of CD49d expression may result in different migrating direction of hematopoietic cell between bone marrow and peripheral blood.