食管癌DNA含量和细胞周期分析的临床意义

Clinical Signification of DNA Content and Cell Cycle Analysis in Human Esophageal Carcinoma

目的 :研究食管癌和癌旁组织DNA含量、细胞周期分布与临床分期及组织病理学分级的关系和意义。方法 :应用流式细胞术 (FCM)测定 4 0例食管癌、癌旁组织、1 0例正常食管粘膜细胞的DNA指数 (DI)、S期细胞百分率 (SPF)、异倍体率。结果 :癌灶中心组织DI、SPF、异倍体率明显高于癌旁组织 ;DI、SPF、异倍体率与年龄、性别无关 ,而随临床分期、组织病理学分级增加而逐渐增加 ;有淋巴结转移的癌组织DI、SPF、异倍体率明显高于无淋巴结转移者。结论 :DNA含量、异倍体率与癌中心灶密切相关 ,SPF可作为组织病理学分级、临床分期的一个参数。DI、SPF、异倍体率与患者的临床信息结合 ,将有助于食管癌患者的诊断。

Objective:To study the relationship and significance between DNA content, cell cycle analysis and histopathology and clinic.Methods:DNA index(DI) and S phase fraction (SPF), DNA aneuploidy rate were measured by flow cytometry(FCM) in 40 cases patients esophageal carcinoma tissue and their correspondent para cancer tissue.Results:DI,SPF and DNA aneyploidy rate in central cancer tissue were higher than in para cancer tissue. These changes were not relation with patients age and sex, but increasing by following clinical stages and histopathological grades increasing. DI,SPF,DNA aneuploidy rate in lymphonodus metastasis tissue were higher than in the no lymphonodus metastasis.Conclusion:DNA content, aneuplidy rate are associated with central cancer tissue.SPF may be useful as an parameter of histopathological grades and clinical stages. They help to evaluate diagnose, treatment and prognosis of esophageal cancer by combined with clinical information of patients.