细胞分裂素在维持紫萍叶状体生命中的重要作用(英文)

Significant Role of Cytokinins in Maintaining the Life of Fronds in Spirodela polyrrhiza

将无根和囊的紫萍 (Spirodelapolyrrhiza)P1 43品系半叶状体分别放在含有和不含 6 苄基嘌呤的培养液上于长日照条件下培养。在不含 6 BA的培养液上培养 4d以后 ,半叶状体开始失绿 ,光合能力降低 ;第 1 2天时 ,半叶状体已接近死亡。但是 ,培养在含有 6 BA的培养液上的半叶状体干重、叶绿素和可溶性蛋白质含量、希尔反应活力、核酮糖 1 ,5 二磷酸羧化酶 /加氧酶的小亚基和其编码基因rbcSmRNA水平显著增加。这些 6 BA处理过的半叶状体平均寿命可达 80d ,几乎等同于完整植物体上叶状体的寿命 ,因此提出细胞分裂素对于紫萍叶状体生物学功能的维持是必需的。

Spirodela polyrrhiza P143 half fronds without roots and pockets were cultivated on liquid medium under long day conditions with or without 6 benzyladenine(6 BA). After 4 d of cultivation on a medium without 6 BA the half fronds began to lose their chlorophyll and decrease in photosynthetic activity, and on the 12th d of cultivation these half fronds were almost dead. But when cultivated on the medium to which 6 BA was added half fronds increased significantly their dry weight, chlorophyll and soluble protein contents, capacity of Hill reaction and the levels of the ribulose 1,5 bisphosphate carboxylase/oxygenase small subunit and the rbcS mRNA encoding it. The 6 BA treated half fronds had a mean longevity of 80 d, almost the same as that of the intact fronds. Therefore, it is proposed that cytokinins are necessary for maintaining the activities of duckweed fronds.