产纤溶酶菌株根霉12#的诱变育种

Breeding of a Rhizopus chinesis producing a fibrinolytic enzyme

以产纤溶酶的华根霉12＃为出发菌株,对其进行紫外线-氯化锂复合诱变,筛选到74株制霉素抗性突变株。所有抗性突变株经进一步固体发酵筛选,获得了4株稳定高产纤溶酶的正突变株,其纤溶酶产量分别比出发菌株提高32.9%、21.5%、22.3%和18.0%。

A Rhizopus chinesis producing a fibrinolytic enzyme is mutagenized with UV combining LiCl and 74 nystatin - resistant mutants are obtained. All mutants are further screened repeatedly through solid - substrate fermentations and then 4 plus -mutants with highly genetic stability are acquired. The productivity of the fibrinolytic enzyme of the plus-mutants increase by 32.9%, 21.5%, 22.3% and 18.0% respectively compared with that of original strain.