高效毛细管电泳-电导检测对四环素衍生物分离测定的研究

Separation and determination of tetracycline compounds by capillary electrophoresis with conductance detection

运用毛细管电泳 电导检测方法对 4种四环素衍生物———土霉素 (OTC)、金霉素 (CTC)、强力霉素 (DOC)和四环素 (TC)的分离进行了研究。在 3.5mmol L三羟基氨基甲烷 (Tris) 7.5mmol L柠檬酸 (Cit)pH 4.0的运行缓冲液中 ,4种四环素衍生物在 1 5min内获得完全分离。四环素衍生物的线性范围分别为 5 .0～5 0 0 μg mLOTC ,3.6～ 42 0 μg mLCTC ,4.5～ 470 μg mLDOC和 2 .5～ 40 0 μg mLTC。检测限 (S N =3)分别为OTC 2 .0 μg mL ,CTC 1 .8μg mL ,DOC2 .5 μg mL和TC 1 .0 μg mL。采用本法对实际样品强力霉素片中强力霉素和土霉素片中土霉素进行测定 ,回收率分别为 97.2 %和 96.4%。

Separation of four tetracycline derivatives: tetracycline(TC), oxytetracycline(OTC), doxycycline(DOC) and chlortetracycline(CTC) were studied by capillary electrophoresis with conductance detection. Satisfactory separation of these four tetracycline compounds was achieved within 15 min by using 3.5 mmol/L Tris-7.5 mmol/L Cit(pH 4.0) as a carrier electrolyte. There were excellent linearity between peak and concentration of analytes in the concentration range of 5.0～ 500 μg/mL for OTC, 3.6～420 μg/mL for CTC, 4.5～470 μg/mL for DOC, 2.5～400 μg/mL for TC. The detection limits (S/N=3) were 2.0 μg/mL, 1.8 μg/mL, 2.5 μg/mL, 1.0 μg/mL for OTC, CTC , DOC, TC, respectively. The recovery rates of DOC and OTC were 97.2% and 96.4%, respectively.