草菇(Volvariella volvacea)、银丝草菇(V.bombycina)原生质体分离研究

STUDIES ON THE ISOLATION OF PROTOPLASTS FROM VOLVARIELLA VOLVACEA AND V.BOMBYCING

本文对草菇、银丝草菇菌丝原生质体制备的最佳条件作了探讨。结果表明,培养两天的草菇菌丝,以0.5MKCI或0.5MMgSO_(4.7)H_2O作渗透压稳定剂,1.5％Lywallzyme(v/m),35℃下酶解1.5—2小时,原生质体产量可达1.5×10~6个/ml以上,培养3天的银丝草菇菌丝,以0.5MKCl作渗透压稳定剂,1.5％Lywallzyme,28℃酶解2—3小时,原生质体产量可达2.8×10~6个/ml以上。此研究对以后的原生质体融合育种打下了基础。

The best conditions for culture of mycelium and isolation of protoplasts on Volvariella volvacea and V.bombycina were studied The results showed, 2-day-old mycelium of V.volvacea, incubated with 1.5% Lywallzyme(v/m), in 0.5M KC1 or 0.5M MgSO4 7H2O at 35℃, the total output of protoplasts could be over 1.5×106/ml within 1. 5- 2hr,while 3- day-old mycelium of V. bombycina, incubated with Lywallzyme(1.5%), in 0.5M KC1 at 28℃, the total number could be more than 2.8×106 /ml within 2-3hr.The results are useful for hybrid-breeding of protoplasts fusion afterwards.