薄叶红厚壳叶化学成分研究(Ⅰ)

CHEMICAL CONSTITUENTS FROM LEAVES OF CALOPHYLLUM MEMBRANACEUM GARDN.

利用硅胶柱层析分离和薄层纯化,从海南薄叶红厚壳(CalophyllummembranaceumGardn.etChamp.)叶的乙醇提取物的乙酸乙酯部分分离得到6个化合物。经理化数据对照和波谱分析,确定它们分别为木栓酮(1),β 谷甾醇(2),海棠果醇(3),异海棠果酸(4),穗花杉双黄酮(5),海棠果酸(6)。其中异海棠果酸在药理实验中发现对肝癌细胞和肺癌细胞具有选择性细胞毒性,IC50值分别为9.074和6.372μg/mL。

Six compounds,namely:friedelin(1),βsitosterol(2),canophyllol(3),isocalophyllic acid(4),amentoflavone(5) and canophyllic acid(6) were isolated from the leaves of Calophyllum membranaceum Gardn.et Champ.Their structures were determined by analysis of 1H NMR,13C NMR and MS.Isocalophyllic acid exhibited selective in vitro cytotoxicity toward Bel7402 and NCI 4460,their IC50 were 9.074 and 6.372 μg/mL,respectively.