摘要
采用农杆菌介导的方法,将嵌合基因PSAG12-IPT导入谷秆两用稻201幼胚诱导的胚性愈伤组织中,经潮霉素筛选,抗性愈伤分化成苗,共获得46个抗性愈伤克隆,126株转基因植株.对这些植株进行PCR检测和GUS染色分析,确定其中80株为阳性植株.
Chimeric gene PSAG12IPT was transformed into embryonic calli induced from grainstrawdualuserice 201 juvenile embryo by Agrobacteriummediated method. After resistance screening of hygromycin and differentiation and growth of plantlets, 46 clones of resistance calli and 126 transgenic plants were obtained. Results of PCR detection and GUS staining analysis showed that 80 of those plants were positive.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2003年第2期205-208,共4页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
福建省科技厅资助项目(2001Z012).
