从赤芝提取的氨基葡聚糖对大鼠实验性溃疡的保护作用(英文)

Protective effects of glycosaminoglycan isolated from Ganoderma lucidum on experimental ulcers in rats

为观察从赤芝提取的氨基葡聚糖对实验性溃疡大鼠的保护作用,采取多步骤从灵芝子实体提取氨基葡聚糖并用色谱法对化学分解产物进行鉴定.大鼠溃疡造型用应激法(冷水中游泳30min)、醋酸法、消炎痛法和幽门结扎法诱导产生.氨基葡聚糖以0.5g·kg-1和1.0g·kg-1剂量溶于灭菌水中备用,氨基葡聚糖溶液在应用诱导剂后立即用胃管灌胃法给药,在冷水游泳和幽门结扎前30min,同法给药.对照组接受相同量的赋形剂,雷尼替丁以100mg·kg-1剂量作阳性对照.结果表明:氨基葡聚糖与雷尼替丁对照同样可以降低胃中氢离子浓度(P<0.001),其预防溃疡发生效应较醋酸法和消炎痛法更为显著;取自灵芝的氨基葡聚糖,是一种具有抗溃疡的活性多糖,其作用机制有待进一步探讨.

To investigate the protective effects of glycosaminoglycans (GAG) from Ganoderma lucidum on experimental ulcers in rats. GAG was isolated from the fruiting body of G. lucidum using a multistep procedure and the resulting extract was identified by chromatographic methods and chemical analyses of hydrolysates. Rat experimental ulcers were induced by stress (swimming in cold water for 30 min), administration of acetic acid or indomethacin, and ligation of gastric pylorus. GAG (0.5 and 1.0 g·kg-1) was dissolved in sterile water and administered by forcefeeding (gastrogarage) immediately before ulcerinducer administration or 30 min before the start of swimming in cold water and ligation of gastric pylorus. Control animals received the same amount of vehicle. Ranitidine (100 mg·kg-1, p.o.) was used positive control. The results indicated that GAG had similar effects on the reduction of the ulcer formation and free gastric H+ levels (P<0.001) compared with ranitidinetreated group. The preventive effects appeared to be more marked in the acetic acid and indomethacintreated groups. These results indicate that GAG isolated from G. lucidum is an active polysaccharide component with ulcerhealing effects. Further studies are required to explore the mechanism of the action of GAG.