激光诱导荧光光谱识别动脉粥样硬化斑块的研究

Laser-induced Fluorescence Spectroscopy Analysis Identifies Atherosclerotic Plaque

采用氩离子激光为激发光源 ,分别研究了离体正常动脉壁、动脉粥样硬化斑块和血液等的激光诱导荧光光谱 结果表明 ,正常动脉壁样品组的荧光光谱强度最大值明显高于动脉粥样硬化斑块样品组 ;斑块的荧光光谱在 5 16nm处存在一个小波谷 ,而正常动脉组织的荧光光谱则无此波谷 ;斑块组织在 5 98nm处与 5 78nm处的荧光强度比值R(I598nm/I578nm)远低于正常动脉壁的比值 ;血红蛋白含量是引起粥样斑块与正常动脉壁的R(I598nm/I578nm)

A study on laser induced fluorescence spectrum is made in human normal arterial samples, atherosclerotic plaques and blood using an argon laser as an excitation light, and the absorption spectra of erythrocyte are made. It shows that there is a noticeable difference between the maximum of fluorescence intensity in plaques and normal arteries wall. The maximum of fluorescence intensity in normal arteries is much more high than in plaques. There are troughs at 516 nm, 546 nm and 578 nm of laser fluorescence spectra in the plaque respectively, however, there are troughs only at 546 nm and 578 nm in normal arterial wall. The ratio R(I 598 nm /I 578 nm ) of fluorescence intensity at 598 nm and 578 nm in plaques is smaller than in normal arteries. These results can be used to discriminate the atherosclerotic plaques from normal arterial tissues. When normal artery wall is rinsed with normal saline for long time, the maximum of fluorescence intensity gradually decreases, and the trough figure of fluorescence spectra becomes shallower and flatter. There is a small absorption peak at 546nm and 578nm in the absorption spectra of erythrocyte. It indicates that the difference of laser fluorescence spectrum between atherosclerotic plaques and normal arterial tissues is directly related to the decrease of erythrocyte content in tissue.