人血管生成素-1原核表达载体的构建与表达

Construction and Expression of Human Angiopoietin 1 Prokaryotic Vector

目的 构建人血管生成素 - 1的原核表达载体 ,并进行表达。方法 利用PCR技术扩出人血管生成素 - 1基因片段 ,并克隆到PQE - 31载体中 ,转化E .coliM 15菌株后进行诱导表达。结果 构建了PQE31Ang - 1原核表达载体 ,在M 15中进行了高效表达。结论 通过原核表达得到人血管生成素 - 1蛋白 ,其具有良好的抗原性 。

Objective To construct and express the human angiopoietin 1 prokaryotic vector.Methods The fragment of human angiopoietin 1 gene was amplified by PCR,cloned into PQE-31 vector,then transformed to E.coli M15 and expressed under the induction.Results The PQE31Ang-1 prokaryotic vector was constructed and effectively expressed in E.coli M15.Conclusion The human angiopoietin 1 protein was obtained in the prokaryotic system and provided with antigen activity.This lays a good foundation of further study on the biological activity and application of human angiopoietin 1.